Nucleotide sequence of the 3′ terminal region of lettuce mosaic potyvirus RNA shows a Gln/Val dipeptide at the cleavage site between the polymerase and the coat protein
Identifieur interne : 000390 ( France/Analysis ); précédent : 000389; suivant : 000391Nucleotide sequence of the 3′ terminal region of lettuce mosaic potyvirus RNA shows a Gln/Val dipeptide at the cleavage site between the polymerase and the coat protein
Auteurs : Sylvie Dinant [France] ; Hervé Lot [France] ; Josette Albouy [France] ; Chantal Kuziak [France] ; Michel Meyer [France] ; Suzanne Astier-Manifacier [France]Source :
- Archives of Virology [ 0304-8608 ] ; 1991-03-01.
English descriptors
- Teeft :
- Amino, Amino acid composition, Amino acid sequence, Amino acids, Antigenic, Antigenicity, Arch virol, Capsid, Capsid protein, Capsid protein gene, Carlavirus groups, Cdna, Cleavage, Cleavage site, Clone, Coat protein, Coat protein gene, Core protein, Dinant, Dougherty, Genome, Homology, Lettuce, Lettuce mosaic virus, Mosaic, Mosaic virus, Nucleic acids, Nucleotide, Nucleotide sequence, Nucleotide sequences, Oligonucleotide, Other potyviruses, Plant viruses, Polymerase, Polyprotein, Potato virus, Potyvirus, Potyvirus group, Potyviruses, Primary structure, Primer, Proc natl acad, Protein, Protein sequences, Putative polymerase, Secondary structure, Sequence analysis, Sequencing, Several potyviruses, Shukla, Sylvie, Sylvie dinant, Terminal region, Virol, Virology, Virus.
Abstract
Summary: DNA complementary to the 3′ terminal 1651 nucleotides of the genome of the common strain of lettuce mosaic virus (LMV-O) has been cloned and sequenced. Microsequencing of the N-terminus enabled localization of the coat protein gene in this sequence. It showed also that the LMV coat protein coding region is at the 3′ end of the genome, and that the coat protein is processed from a larger protein by cleavage at an unusual Q/V dipeptide between the polymerase and the coat protein. This is the first report of such a site for cleavage of a potyvirus polyprotein, where only Q/A, Q/S, and Q/G cleavage sites have been reported. The LMV coat protein gene encodes a 278 amino acid polypeptide with a calculated Mr of 31,171 and is flanked by a region which has a high degree of homology with the putative polymerase and a 3′ untranslated region of 211 nucleotides in length. Percentage of homology with the coat protein of other potyviruses confirms that LMV is a distinct member of this group. Moreover, amino acid homologies noticed with the coat protein of potexvirus, bymovirus, and carlavirus elongated plant viruses suggest a functional significance for the conserved domains.
Url:
DOI: 10.1007/BF01319245
Affiliations:
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ISTEX:6FE64DE8AA04F9DA55D6F4B5A21052A9F9F6F963Le document en format XML
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xml:lang="fr">France</country><wicri:regionArea>Station de Pathologie Végétale, INRA, Montfavet</wicri:regionArea><wicri:noRegion>Montfavet</wicri:noRegion><wicri:noRegion>Montfavet</wicri:noRegion></affiliation></author><author><name sortKey="Albouy, Josette" sort="Albouy, Josette" uniqKey="Albouy J" first="Josette" last="Albouy">Josette Albouy</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Station de Pathologie Végétale, INRA, Versailles</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Versailles</settlement></placeName></affiliation></author><author><name sortKey="Kuziak, Chantal" sort="Kuziak, Chantal" uniqKey="Kuziak C" first="Chantal" last="Kuziak">Chantal Kuziak</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Station de Pathologie Végétale, INRA, Versailles</wicri:regionArea><placeName><region 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type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Versailles</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Archives of Virology</title><title level="j" type="abbrev">Archives of Virology</title><idno type="ISSN">0304-8608</idno><idno type="eISSN">1432-8798</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Vienna</pubPlace><date type="published" when="1991-03-01">1991-03-01</date><biblScope unit="volume">116</biblScope><biblScope unit="issue">1-4</biblScope><biblScope unit="page" from="235">235</biblScope><biblScope unit="page" to="252">252</biblScope></imprint><idno type="ISSN">0304-8608</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0304-8608</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Amino</term><term>Amino acid composition</term><term>Amino acid sequence</term><term>Amino acids</term><term>Antigenic</term><term>Antigenicity</term><term>Arch virol</term><term>Capsid</term><term>Capsid protein</term><term>Capsid protein gene</term><term>Carlavirus groups</term><term>Cdna</term><term>Cleavage</term><term>Cleavage site</term><term>Clone</term><term>Coat protein</term><term>Coat protein gene</term><term>Core protein</term><term>Dinant</term><term>Dougherty</term><term>Genome</term><term>Homology</term><term>Lettuce</term><term>Lettuce mosaic virus</term><term>Mosaic</term><term>Mosaic virus</term><term>Nucleic acids</term><term>Nucleotide</term><term>Nucleotide sequence</term><term>Nucleotide sequences</term><term>Oligonucleotide</term><term>Other potyviruses</term><term>Plant viruses</term><term>Polymerase</term><term>Polyprotein</term><term>Potato virus</term><term>Potyvirus</term><term>Potyvirus group</term><term>Potyviruses</term><term>Primary structure</term><term>Primer</term><term>Proc natl acad</term><term>Protein</term><term>Protein sequences</term><term>Putative polymerase</term><term>Secondary structure</term><term>Sequence analysis</term><term>Sequencing</term><term>Several potyviruses</term><term>Shukla</term><term>Sylvie</term><term>Sylvie dinant</term><term>Terminal region</term><term>Virol</term><term>Virology</term><term>Virus</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: DNA complementary to the 3′ terminal 1651 nucleotides of the genome of the common strain of lettuce mosaic virus (LMV-O) has been cloned and sequenced. Microsequencing of the N-terminus enabled localization of the coat protein gene in this sequence. It showed also that the LMV coat protein coding region is at the 3′ end of the genome, and that the coat protein is processed from a larger protein by cleavage at an unusual Q/V dipeptide between the polymerase and the coat protein. This is the first report of such a site for cleavage of a potyvirus polyprotein, where only Q/A, Q/S, and Q/G cleavage sites have been reported. The LMV coat protein gene encodes a 278 amino acid polypeptide with a calculated Mr of 31,171 and is flanked by a region which has a high degree of homology with the putative polymerase and a 3′ untranslated region of 211 nucleotides in length. Percentage of homology with the coat protein of other potyviruses confirms that LMV is a distinct member of this group. Moreover, amino acid homologies noticed with the coat protein of potexvirus, bymovirus, and carlavirus elongated plant viruses suggest a functional significance for the conserved domains.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Île-de-France</li></region><settlement><li>Versailles</li></settlement></list><tree><country name="France"><region name="Île-de-France"><name sortKey="Dinant, Sylvie" sort="Dinant, Sylvie" uniqKey="Dinant S" first="Sylvie" last="Dinant">Sylvie Dinant</name></region><name sortKey="Albouy, Josette" sort="Albouy, Josette" uniqKey="Albouy J" first="Josette" last="Albouy">Josette Albouy</name><name sortKey="Astier Manifacier, Suzanne" sort="Astier Manifacier, Suzanne" uniqKey="Astier Manifacier S" first="Suzanne" last="Astier-Manifacier">Suzanne Astier-Manifacier</name><name sortKey="Kuziak, Chantal" sort="Kuziak, Chantal" uniqKey="Kuziak C" first="Chantal" last="Kuziak">Chantal Kuziak</name><name sortKey="Lot, Herve" sort="Lot, Herve" uniqKey="Lot H" first="Hervé" last="Lot">Hervé Lot</name><name sortKey="Meyer, Michel" sort="Meyer, Michel" uniqKey="Meyer M" first="Michel" last="Meyer">Michel Meyer</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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